The role of biochemical oxygen demand (BOD) is to measure the amount of oxygen required to remove organic waste from water by bacteria. It should be known that the greater the biochemical oxygen demand, the faster the oxygen in the stream will be depleted. This means there will be less oxygen in the water. Biological oxygen demand is required By providing information to assess the impact of wastewater on the receiving environment. The BOD value is usually expressed in milligrams of oxygen consumed per liter of sample during a 5-day incubation at 20 °C. The purpose is to maintain aquatic life and the aesthetic quality of streams and lakes.